The Molecular Imaging and Nanotechnology Laboratory at the University of Wisconsin - Madison (http://mi.wisc.edu/) is mainly focused on three areas: 1) development of multimodality molecular imaging agents; 2) nanotechnology and its biomedical applications; and 3) molecular therapy of cancer. In this talk, I will present our recent work on molecular imaging and image-guided drug delivery of cancer and other diseases with peptides, proteins, and a variety of nanomaterials. The primary imaging techniques used in these studies are positron emission tomography (PET), photoacoustic tomography (PAT), optical imaging, and magnetic resonance imaging (MRI). Some of the molecular targets that we are investigating are CD105 (i.e. endoglin), PD-1/PD-L1, CTLA-4, CD146, VEGFR, integrin α_vβ₃, among others. The nanomaterials that will be discussed in this presentation may include DNA nanomaterials, nanovaccines, silica-based nanoparticles, micelles, iron oxide nanoparticles, 2-D nanomaterials, hybrid nanomaterials, among many others.